Investigation of Correlation between Expression of Glioma Diphtheria Toxin Receptor and Pathological Grade of Glioma 人脑胶质瘤白喉毒素受体表达水平与其病理级别相关性的探讨
The study on high-density fermentation of E.coli expressing diphtheria toxin mutant CRM197 大肠杆菌表达白喉毒素突变体CRM197高密度发酵工艺研究
Diphtheria is treated with an antitoxin that neutralizes the toxin and produces long-term immunity. 治疗方式为及时注射白喉抗毒素,产生长期的免疫力。
Experiments of Microporous Tangential flow Filtration in Clarification of Diphtheria and Tetanus Culture Suspension and Ultrafiltration in Concertration of Toxin Fluid 应用TFF微孔过滤澄清白喉、破伤风毒素培养液及超滤浓缩的试验
Aim: To construct recombinant expression vector containing transcriptional regulation sequence of human breast cancer DF3/ MUC1 promoter and diphtheria toxin A-chain gene. 目的:构建含人乳癌DF3/MUC1启动子转录调控序列和白喉毒素A链(DTA)基因的重组表达载体。
AIM: To detect dynamically the response of specific antibody secreting cells elicited by a detoxified lipooligosaccharide cross reactive mutant ( dLOS CRM) of diphtheria toxin conjugate vaccine for Moraxella catarrhalis ( M.cat). 目的:动态测定卡他性莫拉氏菌(Moraxellacatarrhalis,M.cat)脱毒脂寡糖(dLOS)蛋白质结合疫苗诱导的抗体分泌细胞的应答状态。
Methods The specific fragment of diphtheria toxin B gene ( toxB) was detected by PCR. PCR amplification products were examined by agarose gel electrophoresis. 方法用PCR扩增白喉杆菌特异的白喉外毒素B基因(toxB)片段,琼脂糖凝胶电泳检测扩增产物。
After adding 0. 02M β- alanine then formalin into purified diphtheria toxin and keeping them for a suitable period, we got such product that was completely detoxified and nontoxicity reverse. 精制白喉毒素加0.02Mβ&丙氨酸,再加甲醛溶液经适当的时间解毒,即可转化为完全类毒化且无毒性逆转的精制白喉类毒素。
Improvement in the detoxication technology for purified diphtheria toxin 精制白喉毒素脱毒工艺的改进
Killing Effect of Expression of the Diphtheria Toxin a Chain Gene Regulated by the EWS-FLI 1 Fusion Gene on Ewings Sarcoma EWS-FLI1介导白喉毒素表达对尤文肉瘤移植瘤杀伤作用研究
Preparation and Characterization of Purified Diphtheria Toxin Standard and Purified Schick Test Toxin 精制白喉标准毒素及精制锡克试验毒素的制备与检定
Inhibitive effect of diphtheria toxin A under control of promoter of human-α fetoprotein gene ( AFP-DTA) using hepatitis B virus envelope as gene transfer vector for hepatocarcinoma cells 以乙肝病毒衣壳为载体的AFP启动子-DTA对肝癌细胞特异杀伤作用的实验研究
Observation on the Effect of Detoxifying Purified Diphtheria Toxin in the Presence of β- Alanine 精制白喉毒素加β&丙氨酸后脱毒效果观察
During the course of detoxification of purified diphtheria toxin by using formalin alone, the average of loss rates of Lf/ ml and Lf/ mg PN were 14.81% and 13%, respectively. 精制白喉毒素单纯用甲醛解毒,解毒过程Lf/ml损失平均为14.81%,Lf/mgPN下降平均为13%;
Results: The expression vector containing transcriptional regulation sequence of human breast cancer DF3/ MUC1 promoter and diphtheria toxin A-chain gene had been successfully constructed and identified by restriction endonuclease and sequencing. 结果:构建了含人乳癌DF3/MUC1启动子转录调控序列和DTA的表达载体,酶切和测序结果与预期完全相符。
AIM: To construct a new recombinant eukaryotic expression plasmid of mouse IFN-γ-inducible protein 10 ( mIP10) gene and a truncated diphtheria toxin ( DT390) gene and investigate its biological function. 目的构建一种新型免疫毒素DT390-mIP10(白喉杆菌毒素390-小鼠γ干扰素诱导性蛋白10)基因的真核表达质粒,并对其功能进行初步研究。
Objective To explore the specific killing effect of diphtheria toxin A-chain ( DTA) gene under control of the transcriptional regulation sequence of DF3/ MUC1 promoter. 目的探讨白喉毒素A链(DTA)基因在DF3/MUC1启动子调控下的杀伤作用。
Expression of diphtheria toxin a chain gene in plants could inhibit their protein synthesis 白喉毒素A链基因在植物中的表达能抑制蛋白质合成
Results 1. The result of restriction enzyme digest and gene sequence showed that the EWS-FLI-1 binding sequence ( ACCGGAAGT) lay at the upper stream of diphtheria toxin A fragment. 结果1、对重组载体的酶切鉴定和测序结果证实,在pS2-DTA中EWS-FLI-1特异性结合序列(ACCGGAAGT)位于白喉毒素A链基因上游。